Importance of Methionine Metabolism in Morula-to-blastocyst Transition in Bovine Preimplantation Embryos
نویسندگان
چکیده
منابع مشابه
Impact of pro-oxidant agents on the morula-blastocyst transition in bovine embryos.
Exposing day 5 bovine morulae to reactive oxygen species induces a delayed degeneration of some blastocysts on day 8 post-insemination (pi) but without affecting the blastocyst rates. The aim of this study was to characterize the resisting and the degenerating population of blastocysts. The kinetics of degeneration of the embryos exposed to the two pro-oxidant agents: 2,2'-azobis (2-amidinoprop...
متن کاملRetinoids during the in vitro transition from bovine morula to blastocyst.
BACKGROUND The conversion of retinol (ROH) to retinoic acid (RA) is crucial during development but has been not studied during blastocyst formation. METHODS AND RESULTS In vitro-produced bovine morulae were treated for 24 h with citral (which inhibits the synthesis of RA from ROH), citral + all trans retinoic acid (ATRA), ATRA or no additives. Citral interfered with blastocyst development, wh...
متن کاملGlucose metabolism of human morula and blastocyst-stage embryos and its relationship to viability after transfer.
The pregnancy rate and implantation rate following blastocyst transfer in the human have been reported to be high; however, it has remained necessary to transfer 2-3 blastocysts to achieve these rates. Morphological criteria are currently used to select blastocysts for transfer and have some limited correlation with ongoing viability. Glucose metabolism of 189 human morula to blastocyst stage e...
متن کاملTransition of cell numbers in bovine preimplantation embryos: in vivo collected and in vitro produced embryos.
The total cell numbers (TCNs) of bovine embryos collected from superovulated donors (VIVO embryos) were counted 0-9 d after ovulation to quantify the developmental process. Using numerical analysis of embryo development, we also compared the developmental process of VIVO embryos, in vitro-fertilized (IVF) embryos and nuclear transfer (NT) embryos obtained from enucleated oocytes and blastomere ...
متن کاملA global view of gene expression in the preimplantation mouse embryo: morula versus blastocyst.
As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. T...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 2012
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.11-096h